Pair Name	Shogaol, Methotrexate
Disease Info	[ICD-11: 2B33.3]	Acute lymphoblastic leukemia		Investigative
Biological Phenomena	Induction-->ROS generation
Gene Regulation	Up-regulation	Expression	BBC3	hsa27113
	Up-regulation	Expression	CDKN1A	hsa1026
	Down-regulation	Expression	FASN	hsa2194
	Up-regulation	Expression	TP53	hsa7157
In Vivo Model	Four‐ to six‐week‐old female athymic nude mice (C57BL/6 nude) were injected with 1.5×10⁷ CCRF‐CEM cells in 100 μL FBS, subcutaneously. In order to confirm leukaemia engraftment, flow cytometry was performed on mice blood samples by using antibodies against CCRF‐CEM cell‐line CD markers.
Result	The current study revealed the in vivo novel anti-leukaemic role of ginger extract, promoted by MTX. Moreover, 6-shogaol was introduced as the major player of ginger cytotoxicity through inducing p53 activity and ROS generation.